comparative analysis of genetic diversity among bt cotton genotypes using est-ssr, issr and morphological markers

Authors

j. ashraf

w. malik

m. iqbal

a. khan

a. qayyum

abstract

robust information of genetic diversity among bt cotton genotypes is still lacking and availability of several marker systems has prompted us to compare their utility for the detection of genetic diversity. here, we report the comparative efficiency of morphological and molecular markers (est-ssrs and issrs) in determining the genetic diversity among 30 pakistani bt cotton genotypes. three different dendrograms based on 20 est-ssrs, 13 issrs and 20 morphological markers divided the 30 bt cotton genotypes into five, six, and three clusters, respectively. est-ssrs and issrs revealed 0.73-1.00 and 0.77-0.97 genetics similarity among bt cotton genotypes which indicated low level of genetic diversity. further population structure analysis showed extensive allelic admixture among bt cotton cultivars and identified three (est-ssrs) and six (issrs) subgroups. the mghes-31 (est-ssrs) and ubc-807 and ubc-815 (issrs) showed maximum values of polymorphic information contents(pic) and dj and had low value of cj. the comparison of two marker systems showed that est-ssrs had high value of expected heterozygosity (hep) and low value of marker index (mi) as compared to issrs. in conclusion, high level of genetic similarity among bt cotton genotypes compel the plant breeders for the introduction of divers/exotic genotypes in their breeding program and marker discriminating indices could be a potential tool for selection of particular marker system to unveil the genetic difference.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Investigation of Genetic Diversity and Structure Analysis of Different Citrus Genotypes Using ISSR Markers

In breeding programs, it is necessary having knowledge of the relatedness and genetic diversity in germplasm pools. The spread of cultivated regions and the high levels of production indicates citrus importance in the global economy. Therefore, 110 citrus genotypes were evaluated using 12 ISSR markers. Overall, 154 polymorphic bands were scored with an average of 12.8 alleles per primer. The po...

full text

Genetic diversity analysis of groundnut genotypes using SSR markers

Groundnut (Arachis hypogaea L.), an important oilseed crop is a rich source of oil and protein. Molecular marker technologies are the effective tools and they are used for the assessment of genetic variability because they are not influenced by the environment. Among the molecular markers, Simple Sequence Repeat (SSR) has proved to be the most powerful tool for variety identification in groundn...

full text

Genetic diversity analysis in Opal cotton hybrids based on SSR, ISSR, and RAPD markers.

Cotton is one of the most economically important crops in Iran; hybridization is a means to increase the genetic diversity and obtain new elite cultivars in this crop. We examined agronomic characteristics and molecular genetic diversity in the Opal cotton (Gossypium hirsutum) cultivar and in F(2) progenies. Ten homo-primers and seven hetero-primers of 26 RAPD primers produced 261 reproduc...

full text

Genetic diversity analysis of Bt cotton genotypes in Pakistan using simple sequence repeat markers.

The popularity of genetically modified insect resistant (Bt) cotton has promoted large scale monocultures, which is thought to worsen the problem of crop genetic homogeneity. Information on genetic diversity among Bt cotton varieties is lacking. We evaluated genetic divergence among 19 Bt cotton genotypes using simple sequence repeat (SSR) markers. Thirty-seven of 104 surveyed primers were...

full text

Assessment of genetic diversity among sunflower genotypes using microsatellite markers

Genetic diversity estimation of plant materials is one of the important pre-breeding activities in breeding field crops. Twenty-one microsatellite markers used to assess genetic diversity and relationship of 68 sunflower genotypes (Helianthus annuus L.). All of 21 pairs of SSR (Simple Sequence Repeats) markers produced a total number of 49 polymorphic bands. DNA fragments ranged from 9...

full text

My Resources

Save resource for easier access later


Journal title:
journal of agricultural science and technology

Publisher: tarbiat modares university

ISSN 1680-7073

volume 18

issue 2 2016

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023